Browse/search for people

Publication - Dr Katherine Williams

    Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase

    Citation

    Yang, X-L, Friedrich, S, Yin, S, Piech, O, Williams, K, Simpson, TJ & Cox, R, 2019, ‘Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase’. Chemical Science, vol 10., pp. 8478-8489

    Abstract

    Exchange of 32 different sub-fragments of the C-methyltransferase (C-MeT), pseudo-ketoreductase (ΨKR) and ketoreductase (KR) catalytic domains of the tenellin iterative Type I polyketide synthase non ribosomal peptide synthetase (PKS-NRPS) TENS by homologous fragments from the desmethylbassianin (DMBS) and militarinone (MILS) PKS-NRPS led to the creation of chimeric synthetases in which programming fidelity was altered, resulting in the production of mixtures of products with different methylation patterns and chain lengths. Swap of KR domain subfragments with the homologous fragments from the KR of the heptaketide militarinone synthetase resulted in the synthesis of penta, hexa and heptaketides. The results of these and previous experiments are rationalised by considering the existence of competition for acyl-carrier protein (ACP) bound substrates between different catalytic domains of the PKS. In particular, competition between the C-MeT and ketoreductase domains (KR) can account for methylation programming, and competition between the KR and the off-loading NRPS accounts for chain-length selectivity.

    Full details in the University publications repository