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Publication - Dr Christy Waterfall

    High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives

    Citation

    Burridge, AJ, Winfield, MO, Allen, AM, Wilkinson, PA, Barker, GL, Coghill, J, Waterfall, C & Edwards, KJ, 2017, ‘High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives’. in: Prem Bhalla, Mohan Singh (eds) Wheat Biotechnology: Methods and Protocols. Humana Press, pp. 293-306

    Abstract

    A lack of genetic diversity between wheat breeding lines has been recognised as a significant block to future yield increases. Wheat breeding and pre-breeding strategies are increasingly using material from wheat ancestors or wild relatives to re-introduce diversity. Where molecular markers are polymorphic between the host and introgressed material, they may be used to track the size and location of the introgressed material through generations of backcrossing. To generate markers for this purpose, sequence capture targeted re‐sequencing was carried out for a range of wheat varieties, wheat relatives and wheat progenitors. From these sequences a large number of putative SNPs were identified and used to generate the Axiom® Wheat HD array. A selection of varieties representing a selection of elite wheat breeding material, progenitor species and wild relatives were used to validate the array. The procedures used are described here in detail.

    Full details in the University publications repository