Vragniau, C, Bufton, JC, Garzoni, F, Stermann, E, Rabi, F, Terrat, C, Guidetti, M, Josserand, V, Williams, M, Woods, CJ, Viedma, G, Bates, P, Verrier, B, Chaperot, L, Schaffitzel, C, Berger, I & Fender, P, 2019, ‘Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display’. Science Advances, vol 5.
Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.
